Dihydrotanshinone-Induced NOX5 Activation Inhibits Breast Cancer Stem Cell through the ROS/Stat3 Signaling Pathway

Figure 4

Effect of DHTS-induced ROS on mammosphere formation. The effect of DHTS on ROS generation was determined using DCF-DA assays and fluorescence microscopy. Effect of DHTS on ROS production in MDA-MB-231 cells by DCF-DA staining. Images were obtained by microscopy at 4x magnification and are representative photos (scale ) (a). Mammospheres were pretreated with/without NAC (10 mM) 1 h prior to treatment with 1 μM DHTS. After treatment for 7 days, mammosphere formation was determined. Images were obtained by using microscopy at 10x magnification and are representative mammospheres (scale ) (b). Effect of the NOX enzyme inhibitor DPI (5 μM) on DHTS-induced mammosphere formation inhibition. Mammosphere formation was determined. The dissociated MDA-231-MB cells were seeded in 6-well plates on CSC media and treated with the indicated concentrations of DHTS and DPI for 7 days (c). Representative images of colonies were recorded. The data shown represent of three independent experiments. vs. the control.

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