Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α₂-Adrenergic Receptor-Dependent Suppression of Oxidative Stress

Figure 5

Silencing of α_2A-AR siRNA erased the protective role of Dex on antioxidation in IEC-6 cells with simulated H/R stimulation. (a) Summary of ROS levels in IEC-6 cells with different treatments. (b) Representative fluorescence immunostaining images of Nrf2 in IEC-6 cells with different treatments. Scale bar, 100 μm. (c–d) Expression of Nrf2, NQO1, and Keap1 proteins and mRNA levels in intestinal tissues. (e–g) Summary of the relative expression levels of the transcripts for the genes HMOX1 (e), SOD1 (f), and CAT (g) in IEC-6 cells with different treatments, for each group. A: control IEC-6 cells; B: IEC-6 cells with H/R treatment; C: IEC-6 cells that were pretreated with 1 nM Dex for 1 h before inducing H/R injury; D: IEC-6 cells with silencing of α_2A-AR siRNA; E: IEC-6 cells with silencing of α_2A-AR siRNA and H/R treatment; F: IEC-6 cells with silencing of α_2A-AR siRNA that were pretreated with Dex prior to H/R injury. , compared to Group A; , compared to Group B; , compared to Group C.

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