Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice

Figure 2

Effect of LMAE on vascular function ex vivo. LMAE (50 mg/kg) was administered daily by oral gavage for 3 weeks, and osmotic minipumps delivering NaCl (0.9%) or angiotensin II (0.5 mg/kg/day) were implanted 1 week after the start of the LMAE pretreatment. The effect of LMAE on Ach-induced vasorelaxation was studied in aortic rings precontracted with U46619 isolated from control mice (a) and Ang II-treated mice (b). Sensitivity to acetylcholine represented by pD2 (c) and maximal effect (Emax) (d) were measured. The same aortic rings were relaxed with SNP, Emax (e); after precontraction with KCl (80 mM), Emax (f). NaCl: mice treated with NaCl, control; NaCl+LMAE: mice treated with LMAE and NaCl; Ang II: mice treated with Ang II; Ang II+LMAE: mice treated with LMAE and Ang II. NaCl and Ang II groups were treated with an equal volume of vehicle (2% of Tween 80). The results are expressed as . -8/group; .

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