Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice

Figure 4

Effects of LMAE on the protein expression of prooxidant enzymes ex vivo. LMAE (50 mg/kg) was administered daily by oral gavage for 3 weeks, and osmotic minipumps delivering NaCl (0.9%) or angiotensin II (0.5 mg/kg/day) were implanted 1 week after the start of the LMAE pretreatment. The protein expression of prooxidant enzymes was measured in isolated aortas. A representative image of the blots is shown in (a). Quantitative evaluation of COX-1 (b), COX-2 (c), NOX-2 (d), Ang II under grey histogram and NOX-4 (e) protein expressions was performed. NaCl: mice treated with NaCl, control; NaCl+LMAE: mice treated with LMAE and NaCl; Ang II: mice treated with Ang II; Ang II+LMAE: mice treated with LMAE and Ang II. NaCl and Ang II groups were treated with an equal volume of vehicle (2% of Tween 80). The results are expressed as . -6/group; .

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