Research Article
Mechanisms of Anthracycline-Enhanced Reactive Oxygen Metabolism in Tumor Cells
Table 2
Requirements for doxorubicin-stimulated superoxide formation in the tumor microsomal fraction. Superoxide production in tumor microsomes was determined in paired, 1 ml reaction mixtures which contained 150 mM potassium phosphate buffer, pH 7.4, 100 μM EDTA, 56 μM acetylated cytochrome c, 200 μg of microsomal protein, and either 0 or 10 μg of SOD. The chemotherapeutic agent was added to the paired reaction mixtures, where specified, before the initiation of the reaction by addition of NADPH (1 mM).
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a.; number in parentheses is number of experiments performed; bN.D. is not detectable; csignificantly different from control (); dsignificantly different from complete system containing NADPH and doxorubicin (); emicrosomes or SOD heated for 60 min in a boiling water bath; samples containing heat-denatured SOD were paired against identical mixtures with native dismutase; fFAD: flavin adenine dinucleotide; FMN: flavin mononucleotide. |