Nicotinic Agonist Inhibits Cardiomyocyte Apoptosis in CVB3-Induced Myocarditis via α3β4-nAChR/PI3K/Akt-Dependent Survivin Upregulation

Figure 2

The involvement of survivin in the antiapoptotic effect of a nicotinic agonist. (a-d) NRC were exposed to CVB3 for 2 h and then treated with nicotine (0, 0.001, 0.01, 0.1, 1, and 10 μM) for 36 h. The total proteins were then collected for western blot. vs. control group. (e-h) NRC were exposed to CVB3 for 2 h and then treated with 1 μM nicotine for different times (24 h, 36 h, 48 h, and 60 h). The control group was cultured for 24 h in the absence of nicotine after CVB3 infection. The total proteins were then collected for western blot. vs. control group.

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