Mitochondrial Transfer of Wharton’s Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts

Figure 3

Mitochondrial transfer from WJMSCs eliminates mutation burden of MELAS fibroblasts with long-term retention. Experimental course is shown in Figure 2(b). (a) Representative result of mutation burden after a separate coculture. (b) Relative proportions of mutation burden. (c) Identification of mitochondrial genotype by sequencing HVR2 segment of mtDNA. Arrows indicate that mtDNA genotype of MF^Hi+WJ is identical to WJ, but not to MF^Hi. significantly different when compared to all experimental groups. WJ: Wharton’s jelly mesenchymal stem cell; MF^Neg: MELAS fibroblast clone harboring negative mutation burden; MF^Hi: MELAS fibroblast clone harboring high mutation burden.

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