Mitochondrial Transfer of Wharton’s Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts

Figure 6

Cell proliferative capacity and mitochondrion-dependent viability are augmented following mitochondrial transfer by WJMSCs. (a, b) cells were seeded in 12-well plates at the day before day 0. Viable cell number was counted with the trypan blue assay at days 0 and 7. A value expressed as percentage relative to Con. (c) Representative image of mitochondrion-dependent viability. Cells were seeded at the day prior to day 0. Glucose-containing culture medium was replaced with glucose-free 10 mM galactose medium at day 0. Cell confluence photographed and viability detected with CCK-8 kit at days 0, 5, and 7. (d) Quantitative results of cell viability. significantly different when compared to control. significantly different when compared to MF^Neg. significantly different between MF^Hi and MF^Hi+WJ. Con: control fibroblast from a normal human; MF^Neg: MELAS fibroblast clone harboring negative mutation burden; MF^Hi: MELAS fibroblast clone harboring high mutation burden.

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