The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network

Figure 2

Many key genes essential for proliferation of cancer cells are selectively regulated upon SOD1 inhibition. (a) Differential mRNA expression in LD100-treated DU145 and RWPE-1 cells displayed as a volcano plot of log₂(fold change) vs. the -log₁₀(padj) for each gene. Genes considered significant below are highlighted in red and green for upregulation and downregulation, respectively. (b, c) Enriched KEGG pathway scatterplots of DEGs in LD100-treated DU145 cells (b) and RWPE-1 cells (c). The scatterplot shows the top 20 signaling pathways with high levels of statistical significance. (d) A Venn diagram showing the overlaps between the differentially expressed genes (DEGs) in LD100-treated DU145 and RWPE-1 cells. (e) An enriched KEGG pathway scatterplot of overlapped 512 DEGs. The scatterplot shows the top 20 signaling pathways with high levels of statistical significance. (f) Heat map of key DEGs in LD100-treated DU145 and RWPE-1 cells. Based on normalized log₁₀(FPKM+1), the heat map is drawn using red and blue for high-expressed genes and low-expressed genes, respectively. (g) Relative FPKM data of KEAP1 in LD100-treated DU145 and RWPE-1 cells. (h) Relative FPKM data of NFE2L2 in LD100-treated DU145 and RWPE-1 cells. (i) A heat map of upregulated antioxidant proteins in representative Nrf2 pathways of LD100-treated DU145 cells. Based on normalized log₁₀(FPKM+1), the heat map is drawn using white and blue for high-expressed genes and low-expressed genes, respectively. Data (g, h) are mean of triplicate (; unpaired Student’s -test), and all error bars are SD.

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