MitoCLox: A Novel Mitochondria-Targeted Fluorescent Probe for Tracing Lipid Peroxidation

Figure 3

Cardiolipin oxidation in liposomes (100 μM) as induced by the addition of 1 μM cytochrome c. Additions are shown by arrows. (a, b) Absorbance spectra of liposomes around 235 nm (a, accumulation of diene conjugates) and around 595 nm (b, oxidation of MitoCLox); the arrow indicates the time of addition of the antioxidant tert-butylhydroquinone (TBHQ, 10 μM). (c, d) Kinetics of accumulation of diene conjugates (c) and of the oxidized form of MitoCLox (d); the arrow indicates the time of addition of the antioxidant tert-butylhydroquinone (TBHQ, 10 μM). (e, f) Effect of MitoCLox (300 nM, the addition is indicated by an arrow) on the rate of accumulation of dienes in cardiolipin liposomes upon stimulation of oxidation by cytochrome c (CytC, 1 μM).

(a)

(b)

(c)

(d)

(e)

(f)