Antioxidant and Cardioprotective Effects of EPA on Early Low-Severity Sepsis through UCP3 and SIRT3 Upholding of the Mitochondrial Redox Potential

Table 2

Real-time PCR primers used in this study.

Gene	Sequence (5-3)
β-Actin	(F) TCTGTGTGGATTGGTGGCTCTA; (R) CTGCTTGCTGATCCACATCTG
HIF-1	(F) AAGCACTAGACAAAGCTCACCTG; (R) TTGACCATATCGCTGTCCAC
HK2	(F) GAGCTACCACGCACCCTAC; (R) CACCCTTACTCGGAGCACAC
HPRT	(F) TTGCTGACCTGCTGGATTAC; (R) AGTTGAGAGATCATCTCCAC
IL-1β	(F) CACCTTCTTTTCCTTCATCTTTG; (R) GTCGTTGCTTGTCTCTCCTTGTA
LDHH	(F) ACCCCGTGTCTACAATGGTG; (R) GGTTGATGACGCTGGTCAGT
LDHM	(F) TGAAGTCTCTGAACCCGCAG; (R) CGTATGCACTGTCAACCACC
PDK4	(F) CACACCTTCACCACATGCTC; (R) CGTGAATTGTCCATCACAGG
PFK2	(F) TTGCGAGGAAAGCAGTTTGC; (R) ACACGCCAGCATCAATCTCA
PKm	(F) GTCAATCCACAGACCCCCTG; (R) CCACTTGGTGAGCACTCCTG
SIRT3	(F) TGCTACTCATTCTTGGGACCTC; (R) CTGTACCGATTCAGACAAGCTG
SOD1	(F) CAGGACCTCATTTTAATCCTCAC; (R) TGCCCAGGTCTCCAACAT
SOD2	(F) CCGAGGAGAAGTACCACGAG; (R) GCTTGATAGCCTCCAGCAAC
TNF-α	(F) GCCTCTTCTCATTCCTGCTC; (R) GAGCCCATTTGGGAACTTCT

(F): forward; (R): reverse.