miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway

Figure 4

The effects of LATS2 expression on A549 cell proliferation, migration, and invasion. (a) Colony formation of A549 cells transfected with LATS2, miR-25, or the combination of both miR-25 and LATS2 plasmids. (b, c) Wound healing and transwell assays for migration and invasion capacities of A549 cells transfected with miR-25 and/or LATS2. (d) Western blot assays for LATS2, downstream effectors of the Hippo pathway (phosphorylated YAP and YAP), and metastasis-related proteins (E-cadherin, Vimentin, and MMP9) in A549 cells transfected with miR-25 and/or LATS2. Results were presented as the . (e) Immunofluorescence assay for nuclear translocation of YAP protein. Representative immunofluorescent images of YAP staining were shown in the left panel (green: YAP; blue: nuclear; scale bar, 10 μm). The nuclear localization of YAP is quantified in the right panel by ImageJ software. , , and .

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