miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway

Figure 5

miR-25 antagomir inhibits lung cancer growth and metastasis in mouse xenografts. (a) Time course of tumor volumes from immunodeficient mice treated with the miR-25 antagomir or a control. Results were presented as the . and . (b, c) The image and the weight of xenograft tumors one month after the treatment. was shown. . (d) Representative images of mouse lungs and livers. (e) Histological morphology of mouse lungs and livers by HE staining. The magnification of the upper row images was 40x, . The magnification of the bottom row images was 400x, . (f, h) The incidence of lung and liver metastasis in mice after tail intravenous injections with the miR-25 antagomir was shown in the table. (g) Quantification of the metastasis nodules in the lungs of each group (). . (i) Representative images of immunohistochemical analysis of LATS2, E-cadherin, Vimentin, and MMP9 in lung metastasis (magnification, 400x. ). Brown represented the target protein in IHC, and blue represented nuclear staining.

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