Stem Cell-Derived Exosomes Prevent Aging-Induced Cardiac Dysfunction through a Novel Exosome/lncRNA MALAT1/NF-κB/TNF-α Signaling Pathway

Figure 3

H₂O₂ induces senescence in cardiomyocytes. (a) The mRNA and protein expression levels of p21 was increased in H9C2 cells treated with 200 μM H₂O₂ for 12 h. (b) Immunohistochemistry staining of β-gal in cardiomyocytes treated with H₂O₂. (c) Edu staining for cell proliferation. (d) TNF-α mRNA levels were determined by real-time PCR, and TNF-α protein levels were analyzed by Western blot. (e) Dual luciferase assay revealed increased NF-κB activity in H₂O₂-treated cells. (f) Western blot and (g) flow imaging revealed increased expression of p-p65 in H₂O₂-treated cells. /group. and .

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