Stem Cell-Derived Exosomes Prevent Aging-Induced Cardiac Dysfunction through a Novel Exosome/lncRNA MALAT1/NF-κB/TNF-α Signaling Pathway

Figure 4

Inactivation of the NF-κB signaling pathway prevents H₂O₂-induced cell senescence. (a) Dual luciferase assay revealed increased NF-κB activity in H₂O₂-treated cells, and IMD-0354 (IMD), a specific IKKβ inhibitor, inhibited H₂O₂-induced NF-κB activation. (b) IMD inhibited H₂O₂-induced expression of p-p65 in cardiomyocytes. (c) IMD inhibited H₂O₂-induced TNF-α mRNA and protein expression. (d) IMD inhibited H₂O₂-induced p21 mRNA and protein expression. (e) Transfection of cardiomyocytes with siRNA targeting IKKβ (siIKKβ) resulted in reduction of IKKβ protein expression. (f) IKKβ silencing in cardiomyocytes led to reduced expression of p-p65. (g) IKKβ silencing in cardiomyocytes led to reduced expression of TNF-α. (h) IKKβ silencing in cardiomyocytes led to reduced expression of p21. /group, , , and .

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