Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids and Insulin on Muscle Protein Synthesis

Figure 3

The glucocorticoid receptor mediates the inhibitory effects of DEX on protein synthesis and CSE expression. Myoblasts were preincubated with or without RU 486 (100 nM) for 30 min. Cells were then incubated with or without DEX (10 μM) for 6 h, after which they were incubated with puromycin (10 μM) for 30 min to measure protein synthesis rates. Cell lysates were immunoblotted with specific antibodies. (a) Protein synthesis was measured with an antibody against puromycin, (b) CSE and CBS levels were detected by immunoblotting using CSE and CBS antibodies, (c) antibodies against mTOR or p-mTOR (Ser 2448) were used to detect the protein levels, and (d) antibodies against p70S6K or p-p70S6K (Thr 389) were used to detect the protein. The data are presented as the (); ; .
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