Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 4

Research Article

Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids and Insulin on Muscle Protein Synthesis

Figure 4

L-cysteine stimulated protein synthesis in chicken myoblasts via H2S. Myoblasts were incubated with or without L-cysteine (1 mM) and treated with or without DEX (10 μM) for 6 h (a, b, c, and d). Alternatively, the cells were treated with or without PAG (10 mM) for 6 h (e, f, g, and h). After all treatments were complete, cells were incubated with puromycin (10 μM) for 30 min to evaluate protein synthesis rates. The cell lysates were immunoblotted with specific antibodies. (a, e) Blots were incubated with an antibody against puromycin, (b, f) antibodies against CSE and CBS, (c, g) antibodies against mTOR or p-mTOR (Ser 2448), and (d, h) antibodies against p70S6K or p-p70S6K (Thr 389). The data are presented as the (); ; .

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