Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 5

Research Article

Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids and Insulin on Muscle Protein Synthesis

Figure 5

H2S stimulated protein synthesis in chicken myoblasts. Myoblasts were incubated with or without NaHS (500 μM). Cells were simultaneously treated with or without DEX (10 μM) for 6 h. (a) The concentration of H2S in culture media supplemented with NaHS at different times (10, 30, 60 min, or 6 h). (b) Protein synthesis was measured with an antibody against puromycin. (c) CSE and CBS levels were detected by immunoblotting using CSE and CBS antibodies. (d) The total and phosphorylated mTOR levels were analysed with antibodies against mTOR and p-mTOR (Ser 2448), respectively; (e) the total and phosphorylated p70S6K levels were analysed using antibodies against p70S6K or p-p70S6K (Thr 389), respectively. The data were presented as the (); ; ; .

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.