Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 8

Research Article

Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids and Insulin on Muscle Protein Synthesis

Figure 8

CSE mediated the stimulatory effects of insulin on protein synthesis and the activity of the mTOR/p70S6K signalling pathway. Myoblasts were incubated with insulin (5 μg/mL) for 6 h and treated with or without PAG (10 mM). All cells were then incubated with puromycin (10 μM) for 30 min to evaluate protein synthesis rates. Cell lysates were immunoblotted with the following specific antibodies: (a) puromycin, (b) CSE and CBS, (c) mTOR or p-mTOR (Ser 2448), and (d) p70S6K or p-p70S6K (Thr 389). The data are presented as the (); ; .

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