Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells

<div>Isoliquiritigenin induces apoptosis in A375 melanoma cells. (a) Cell proliferation rate was detected by the MTT assay. (b) Hoechst 33258 staining showing nuclear shrinkage in A375 cells. (c) Phase-contrast micrographs (200x) showing morphological changes in isoliquiritigenin- (ISL-) treated A375 cells. (d) Apoptosis was measured by flow cytometry using double stain with annexin V and propidium iodide in A375 cells treated with ISL. (e) Western blotting analysis for cleaved PARP and cleaved caspase-3. (f) Analysis grayscale of band for western blotting. 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Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells 

Figure 1 | Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells 