Research Article
Melanogenic Effects of Maclurin Are Mediated through the Activation of cAMP/PKA/CREB and p38 MAPK/CREB Signaling Pathways
Figure 7
Maclurin protected human epidermal melanocytes against the H2O2-mediated decrease in cell viability. (a) The BrdU incorporation assay was used to measure the percentage of viable cells 14 h after H2O2 treatment. vs. control group, o vs. H2O2 (150 μM)-treated control. (b) Effects of maclurin on UVB-induced ROS production in human epidermal melanocytes. ROS-induced dichlorofluorescein (DCF) formation was measured using a spectrophotometer. vs. control group, o vs. UVB-irradiated control. (c) ROS-induced DCF fluorescence was visualized by fluorescence microscopy. vs. control group, o vs. UVB-irradiated control. (d, e) Effects of maclurin on the H2O2-induced reduction of MITF protein and melanin content in human epidermal melanocytes. vs. control group, o vs. H2O2-treated control. SB: SB203580; M: maclurin.
| (a) |
| (b) |
| (c) |
| (d) |
| (e) |