Hyperglycemia-Induced Oxidative Stress Abrogates Remifentanil Preconditioning-Mediated Cardioprotection in Diabetic Rats by Impairing Caveolin-3-Modulated PI3K/Akt and JAK2/STAT3 Signaling
Cardiac-specific knockdown of Cav-3 expression abolished RPC cardioprotection in NAC-treated diabetic rats. NAC-treated diabetic rats were transfected with AAV9-Cav-3 shRNA for 3 weeks; then, the rats were subjected to myocardial I/R. (a–e) Representative Western blot of Cav-3 compared with GADPH (a), infarct size (b), plasma CK-MB (c), p-Akt (ser473) compared with total Akt (d), and p-STAT3 (Tyr705) compared with total STAT3 (e). All the results are expressed as ,. Differences were determined by one-way analysis of variance (ANOVA) followed by Tukey’s test. vs. the diabetes+NAC+I/R group; vs. the diabetes+NAC+RPC group.