Products of Sulfide/Selenite Interaction Possess Antioxidant Properties, Scavenge Superoxide-Derived Radicals, React with DNA, and Modulate Blood Pressure and Tension of Isolated Thoracic Aorta

Figure 4

Influence of the preincubation time of the H₂S/SeO₃²⁻ mixture on the time-dependent reduction of ^⋅cPTIO. The reduction of ^⋅cPTIO (100 μmol L⁻¹) once added into the preincubated H₂S/SeO₃²⁻ mixture (100/50 in μmol L⁻¹, (a, b); 100/100 in μmol L⁻¹ (c, d)) was evaluated as the decrease of absorbance at 358 nm minus 420 nm (a, c) and the decrease of absorbance at 560 nm (b, d), respectively. The preincubation time of the H₂S/SeO₃²⁻ mixture was 0-40 min (see legend). (e) Impact of the preincubation time of the H₂S/SeO₃²⁻ mixture on the reduction of ^⋅cPTIO. ^⋅cPTIO (100 μM) was added into preincubated H₂S/SeO₃²⁻ (in μmol L⁻¹, 100/100, circles; 100/50, triangles; 100/25, diamonds) at pH 7.4, 37°C. Data represent the reduction of ^⋅cPTIO in 1^st minute after addition of ^⋅cPTIO.

(a)

(b)

(c)

(d)

(e)