Products of Sulfide/Selenite Interaction Possess Antioxidant Properties, Scavenge Superoxide-Derived Radicals, React with DNA, and Modulate Blood Pressure and Tension of Isolated Thoracic Aorta

Figure 6

The influence of H₂S/SeO₃²⁻ on pDNA cleavage in the absence and presence of H₂O₂ and DMSO. Representative gels (a1, b1) and column graphs (a2, b2) indicating the effects of increasing concentrations of SeO₃²⁻ (0-1 mmol L⁻¹) on pDNA cleavage in the presence of 1 mmol L⁻¹ H₂S, without (a1, a2) and with 1 mmol L⁻¹ H₂O₂ (b1, b2). The band at the bottom corresponds to the circular supercoiled form of pDNA, and the less intense band appearing above, in the case of Fe²⁺-H₂O₂, represents the linear form of pDNA. The top band corresponds to the nicked circular form of pDNA. The effects of 150 μmol L⁻¹ FeCl₂ + 1 mmol L⁻¹ H₂O₂ (full column) are shown for comparison. Values are the , . Representative gels (c1) and column graph (c2) showing the effects of increasing concentrations of DMSO on H₂S/SeO₃²⁻ (0.25/1 in mmol L⁻¹)-induced pDNA cleavage. DMSO concentrations: () DMSO; ; ; ; . Values represent , .

(a)

(b)

(c)