Products of Sulfide/Selenite Interaction Possess Antioxidant Properties, Scavenge Superoxide-Derived Radicals, React with DNA, and Modulate Blood Pressure and Tension of Isolated Thoracic Aorta

Figure 7

Time-dependent tonus of isolated thoracic aorta. The original records of changes in NA (1 μmol L⁻¹)-increased arterial tone of isolated rat thoracic aorta after addition of SeO₃²⁻ (100 μmol L⁻¹), H₂S (200 μmol L⁻¹), and H₂S/SeO₃²⁻ (200/100 in μmol L⁻¹) mixture. The arrow indicates the compound application (a). The effects of SeO₃²⁻, H₂S, and H₂S/SeO₃²⁻ on NA (1 μmol L⁻¹)-precontracted rings of rat thoracic aorta. The rings were exposed to bolus dose of H₂S (200 μmol L⁻¹, relaxation), SeO₃²⁻ (100 μmol L⁻¹, nonsignificant contraction), and that of the mixture H₂S/SeO₃²⁻ (100 μmol L⁻¹ of SeO₃²⁻ immediately followed by 200 μmol L⁻¹ H₂S). The SeO₃²⁻/H₂S mixture had a biphasic activity; firstly, it significantly relaxed the aorta, which was followed by significant contraction (b). Asterisks mark the statistical significance of H₂S/SeO₃²⁻ mixture vs. H₂S (, ).

(a)

(b)