Products of Sulfide/Selenite Interaction Possess Antioxidant Properties, Scavenge Superoxide-Derived Radicals, React with DNA, and Modulate Blood Pressure and Tension of Isolated Thoracic Aorta

Figure 8

The time-dependent effects of SeO₃²⁻, H₂S, and H₂S/SeO₃²⁻ on rat BP and pulse BP. Representative traces of the time-dependent effect of i.v. bolus administration of H₂S (10 μmol kg⁻¹; (a, d)) and its mixture with 5 μmol kg⁻¹ SeO₃²⁻ prepared at pH ~ 7.4 (b, e) and pH ~ 11 (c, f) solution on BP (a, b, c) and pulse BP (d, e, f). Transient changes of rat BP (g) and pulse BP (h) after i.v. bolus administration of SeO₃²⁻ (5 μmol kg⁻¹, empty column), H₂S (10 μmol kg⁻¹, empty coarse column) and their mixture (SeO₃²⁻/H₂S, 5/10 in μmol kg⁻¹) prepared at pH ~ 7.4 (grey column) and pH ~ 11 (grey coarse column). Data are presented as ; . To test a statistical significance between group differences, we used one-way ANOVA followed by Dunnett’s test for multiple comparisons. Hence, we also observed the biphasic effect of Na₂S in our previous study [54]; we compared a set of “first part” and “second part” effects of SeO₃²⁻, H₂S/SeO₃²⁻ at pH ~ 7.4, and H₂S/SeO₃²⁻ at pH ~ 11.0 to the corresponding effect of Na₂S on systolic or pulse blood pressure. Only the mixture of H₂S/SeO₃²⁻ prepared at pH ~ 11.0 was able to generate similar decrease and subsequent increase or vice versa in systolic blood pressure or pulse blood pressure as the H₂S, respectively. Asterisks mark the statistical significance as follows: , , and .