Cholic Acid Protects In Vitro Neurovascular Units against Oxygen and Glucose Deprivation-Induced Injury through the BDNF-TrkB Signaling Pathway

Figure 1

Experimental steps of this study. Neurons were cultured at the bottom of the Transwell filter at 0 h. After 44 h, astrocytes were seeded on the upper side of the inserts. After 52 h, BMECs were seeded on the inner side of the inserts. Experiments were performed after 120 hours of coculture. Based on the establishment of the in vitro NVU system, structural and functional evaluations under physiological conditions were undertaken at 172 h. The cells were treated with CA for 24 hours prior to OGD/R. To mimic ischemic conditions, cocultures were subjected to OGD for 1 h, and the cultures returned to the normoxic incubator for 24 h. The structural and functional evaluations under pathophysiological conditions were undertaken at 221 h.