Cholic Acid Protects In Vitro Neurovascular Units against Oxygen and Glucose Deprivation-Induced Injury through the BDNF-TrkB Signaling Pathway

Figure 2

The BBB function and neuronal biological behaviors in the NVU model. (a) Neurons were positive for MAP2 (red). (b) Astrocytes were positive for GFAP (green). (c) BMECs were positive for VWf (red). (d) TEER was continuously tested within 7 d in BMECs: B group (B), B+N group (B+N), B+A group (B+A), and B+A+N group (B+A+N). (e) Immunostaining for ZO-1 (green, arrow) in B, B+N, B+A, and B+A+N. (f) Expression of tight junction protein (ZO-1, occludin, and claudin-5) in B, B+N, B+A, and B+A+N. (g) The permeability coefficient of SF in B, B+N, B+A, and B+A+N. (h) Expression of γ-GT in B, B+N, B+A, and B+A+N. (i) Neuronal morphology in N, B+N, A+N group (A+N), and B+A+N. (j) Expression of MAP2 in N, B+N, A+N, and B+A+N. Quantified results were normalized to β-actin expression. vs. the B+A+N group.

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