Secoisolariciresinol Diglucoside Delays the Progression of Aging-Related Diseases and Extends the Lifespan of Caenorhabditis elegans via DAF-16 and HSF-1

Figure 3

SDG improves the oxidative stress resistance of C. elegans. (a) The measurement of reactive oxygen species (ROS). Worms were observed and photographed using a fluorescent microscope (Leica DM6B) with appropriate filter sets for GFP (magnification: 100x). The quantification of fluorescence intensity showed that SDG could decrease the level of ROS in worms (, two-tailed -test). (b) The survival curve of wild-type C. elegans (N2) treated with or without 500 μM of SDG under oxidative stress induced by 20 mM of paraquat. SDG can significantly extend the lifespan of C. elegans by up to 27.0% (, log-rank test). (c) The survival of hsf-1 mutant strain PS3551 treated with or without 500 μM of SDG at 20°C. SDG could not further extend the lifespan of hsf-1 mutant. A statistically significant value was calculated by the -test or log-rank test; ^★★★. The results from repeated experiments and the statistical details are summarized in supplementary Tables S5 and S8.

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