ROS-Mediated Necroptosis Is Involved in Iron Overload-Induced Osteoblastic Cell Death

Figure 2

Iron overload-induced necrotic cell death in the osteoblastic cells. (a) Representative data were analyzed using the flow cytometer after Annexin-V/PI staining in osteoblastic cells after exposure to 50-200 μM FAC for 120 h. In each plot, the lower left quadrant (Annexin-V-/PI-) corresponds to live cells, the lower right quadrant (Annexin-V+/PI-) corresponds to apoptosis, and the upper left and right quadrants (Annexin-V-/PI+ and Annexin-V+/PI+) correspond to necrotic cell. (b) Histogram statistical analysis illustrated the dose-dependent increase of PI-positive osteoblasts. Values are expressed as the from three independent experiments ( vs. saline control, ANOVA test). (c) Representative confocal photomicrograph of Hoechst 33258/PI staining. After treatment with FAC (50-200 μM) for 120 h, the osteoblastic cells were counterstained with Hoechst 33258 and PI. PI positive represents necrotic cells. .

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