Research Article
ROS-Mediated Necroptosis Is Involved in Iron Overload-Induced Osteoblastic Cell Death
Figure 6
Iron overload induces the production of ROS in osteoblastic cells. (a) Representative data were analyzed using a flow cytometer after H2DCF-DA staining in osteoblasts after exposure to FAC (50-200 μM) for 120 h. (b) Histogram statistical analysis illustrated the production of ROS in osteoblasts. Values are expressed as the from three independent experiments ( vs. saline control, ANOVA test). (c) Representative fluorescence photomicrograph was obtained in situ H2DCF-DA staining by the fluorescence microscope. .
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