Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway

Figure 3

AZA promoted AML cell apoptosis. (a) HL60 cells were treated with 5.0 mM AZA for 24 h. The collected cells were stained with JC-1 dye, and the MMP was analyzed by flow cytometry. (b) U937 cells were treated with 5.0 mM AZA for 24 h. The collected cells were stained with Annexin V and PI. Cell apoptosis rate was analyzed by flow cytometry. (c) Cell cycle was analyzed by flow cytometry after U937 cells were treated with 5.0 mM AZA for 24 h. (d) Cell apoptosis rate was analyzed by flow cytometry after healthy PBMCs were treated with 5.0 mM AZA for 24 h. Each experiment was repeated three times, and . ns: no significance.

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