Astragalus membranaceus Injection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Figure 3

Akt/mTOR mediated the autophagy inhibition caused by LPS in ANA-1 cells. Western blot analysis of the changes in the levels of p-mTOR, p-Akt, LC3, and p62 proteins in ANA-1 cells treated with (a) varying doses of LPS and (b, d) 50 μM LY294002+1 μg/mL LPS and 250 nM Torin 1+1 μg/mL LPS. β-Actin was used as the control. (c) Concentration of IL-6 in the culture supernatant detected by ELISA. Data represent (). Asterisks () indicate significant differences compared with the control group (, ). Number signs (#) indicate significant differences compared with the LPS group (^#, ^##).

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