Astragalus membranaceus Injection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Figure 4

AM can mitigate the inhibitory effect of LPS on autophagy in ANA-1 cells. (a, c) Green-stained LC3 and green-stained p62 of ANA-1 cells in different groups as shown in the immunofluorescence assay (FITC-labeled goat anti-mouse IgG, LC3 antibody and p62 antibody for basement antibody). (b) Percentage of LC3 punctated cells. (e–g) Western blot analysis of the changes in the levels of LC3 and p62 proteins in ANA cells induced by different doses of AM at different coincubation times with or without LPS. β-Actin was used as the control. Data represent (). Asterisks () indicate significant differences compared with the control group (, ). Number signs (#) indicate significant differences compared with the LPS group (^#, ^##).

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