Astragalus membranaceus Injection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Figure 7

siRNA AMPK reversed the AM-induced autophagy and inhibition of IL-6 production in ANA-1 cells. (a) AMPK siRNA reduced the level of p-AMPK, (b) whereas increased the level of IL-6, in the cell. (c) Western blot analysis of the effects of AM on AMPK, Beclin 1, LC3, p-AMPK, and IL-6 proteins in ANA-1 cells before and after knocking down AMPK. β-Actin was used as the control. Data represent (). Asterisks () indicate significant differences compared with the control group (, ). Number signs (#) indicate significant differences compared with the LPS group (^#, ^##).

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