The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo

Figure 1

The effect of coculture of osteoblasts and osteoclasts on cell differentiation with or without Ti. ALP staining and TRAP staining under conditions of direct coculture of BMMs and 3T3-E1 cells with or without Ti (a–d). (e–l) Show ALP staining and Alizarin Red staining (AR) for simulated coculture with or without Ti. (m–p) Show TRAP staining for simulated coculture with or without Ti. (q) Shows a quantitative analysis of ALP staining. (r) Shows the effect on osteoblast differentiation under coculture conditions. (s, t) Show a quantitative analysis of the number and area of osteoclasts. Data are represented as , (, ).

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