Hydrogen Sulfide Promotes Cardiomyocyte Proliferation and Heart Regeneration via ROS Scavenging

Figure 3

NaHS promotes heart regeneration related to CM proliferation. (a) Schema of the animal experiment. (b–d) Representative images and statistics of echo analysis 21 days post MI. LVEF: left ventricular ejection fraction; LVFS: left ventricular fraction shortening. (e) Representative images of Masson’s trichrome-stained heart sections from mice 21 days post MI. (f) Neonatal mouse heart regeneration percentages. (g) LVAWd: left ventricular anterior wall diastolic thickness. Vehicle: ; NaHS: . (h–k) Representative images and related statistical results of CM mitosis and cytokinesis, as indicated by pH3, Ki67, and Aurora B staining. Actinin was used to label CMs, and DAPI was used to label nuclei. Vehicle: ; PAG: . Scale . (l) Cell size was measured by WGA staining. Actinin was used to label CMs, and DAPI was used to label nuclei. Scale . Vehicle: ; PAG: . The data are presented as the . and by Student’s -test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)