Salvianolic Acid B Improves Postresuscitation Myocardial and Cerebral Outcomes in a Murine Model of Cardiac Arrest: Involvement of Nrf2 Signaling Pathway

Figure 5

Salvianolic acid B treatment suppressed the superoxide production in the heart following cardiac arrest and cardiopulmonary resuscitation (CA/CPR). (a) ROS steady-state levels in the myocardium were determined by in situ detection of superoxide with dihydroethidium fluorescence among different groups ( per group). (b) NADPH oxidase activity was assessed using a lucigenin assay 3 hours following CA/CPR ( per group). (c, d) gp91^phox expression was determined using Western blot analysis. GAPDH was used as a loading control and results were converted to the fold change relative to the sham-operated group ( per group). Statistical significance was determined using one-way ANOVA followed by post-hoc Bonferroni correction. ^## vs. sham-operated controls; or vs. vehicle group. ROS: reactive oxygen species; DHE: dihydroethidium; NADPH: nicotinamide adenine dinucleotide phosphate.

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