Salvianolic Acid B Improves Postresuscitation Myocardial and Cerebral Outcomes in a Murine Model of Cardiac Arrest: Involvement of Nrf2 Signaling Pathway

Figure 7

Knockdown of Nrf2 abolished the antioxidative and antiapoptotic effects of Sal B treatment in vitro. (a, b) Protein expression of Nrf2 was assessed using immunoblot 72 hours after Nrf2 siRNA or NC siRNA transfection in H9c2 cardiomyocytes. ^## vs. NC siRNA. (c) Representative fluorescent images of DHE and TUNEL staining in the presence of Nrf2 siRNA or NC siRNA with or without Sal B treatment (10 μM) in H9c2 cardiomyocytes after H/R insult. (d) Quantification of the number of TUNEL-positive cells in the indicated groups. (e, f) gp91^phox expression was determined using Western blot analysis. (g) Western blot analysis of Bax, Bcl-2, and cleaved caspase-3 from the indicated groups. (h) The ratio of Bcl-2/Bax was determined from the Bcl-2 and Bax protein band densities and converted to fold change relative to Blank. (i) Quantitative value of protein expression levels of cleaved caspase-3. GAPDH served as the internal control and results were converted to fold change relative to Blank. ^# or ^## vs. Blank; or vs. NC+Sal B. NC: negative control siRNA; H/R: hypoxia/reoxygenation.

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