Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury

Figure 1

ROS mediated iPSC improvement of renal morphology and function. (a) C57BL/6 male mice were subjected to 50 min of kidney ischemia followed by 24 h and 48 h reperfusion, respectively. PBS, iPSCs, or iPSCs pretreated with H₂O₂ or NAC were injected via the tail vein. Kidney tissues were fixed, embedded, sectioned, and stained with H&E. Histological changes included tubular cell necrosis, cytoplasmic vacuole formation, hemorrhage, and tubular dilatation were assessed with a maximum score of 4. (b) Semiquantitative analysis of morphological changes. (c) Serum creatinine (μmol/L) and (d) blood urea nitrogen (BUN; mg/dL) were measured at 24 h and 48 h after reperfusion; . (e) Glomerular filtration rate measured by transcutaneous decay of FITC-sinistrin; .

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