Research Article
Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury
Figure 2
ROS promoted while NAC inhibited iPSC trafficking to the injured kidney. (a) Quantitative PCR analysis of Tet-on and GAPDH genes in the injured kidney. Tet-on was detected in the kidneys of mice receiving iPSCs with or without H2O2 or NAC pretreatment but not from mice receiving PBS or undergoing sham operations. Relative amount of Tet-on DNA over GAPDH DNA in the injured kidney; . (b, c) Injected PKH26-labeled iPSCs were not positive for makers of proximal (b) or distal (c) tubular cells. Red: injected iPSCs. Green: proximal (b) or distal (c) tubular cells; .
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