Intracellular Reactive Oxygen Species Mediate the Therapeutic Effect of Induced Pluripotent Stem Cells for Acute Kidney Injury

Figure 5

ROS regulated iPSC viability, adhesion, migration, and mitochondrial bioenergetics. (a) Temporary treatment (2 h) of iPSCs with ROS or NAC did not affect iPSC viability (). (b) Persistent treatment (24 h) of iPSCs with ROS and NAC decreased iPSC viability (). (c) ROS pretreatment promoted while NAC pretreatment inhibited iPSC migration through porous membranes (). (d) ROS pretreatment promoted while NAC pretreatment inhibited iPSC adhesion to extracellular matrix-coated culture surfaces (). (e) Basal oxygen consumption rate, ATP production, maximal respiration rate, and proton leak respiration of iPSCs, H₂O₂-treated iPSCs, or NAC-treated iPSCs ().

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