Research Article
Metformin Protects ARPE-19 Cells from Glyoxal-Induced Oxidative Stress
Figure 7
Metformin upregulated tight junction proteins in glyoxal-treated ARPE-19 cells. Glyoxal-induced ARPE-19 cells were cotreated with or without metformin (0.1 mM) for 24 h. Western blot and quantification of occludin (a) and ZO-1 (b). (c) Examination of ZO-1 immunostaining by confocal microscopy. Data are expressed as the ( in (a), in (b), versus glyoxal group). NC: negative control; N: normal control; G: glyoxal-treated group; G+M: glyoxal and metformin cotreated group. Scale bar, 25 μm.
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