Capsaicin Alleviates the Deteriorative Mitochondrial Function by Upregulating 14-3-3η in Anoxic or Anoxic/Reoxygenated Cardiomyocytes

Figure 3

Cap reduces ROS generation by maintaining the redox balance following anoxia or A/R injury. (a, b) Fluorescent probe DCFH-DA indicating ROS level was detected by flow cytometry and column chart of average fluorescence intensity values during A/R exposure. (c, d) Mitochondrial NAD levels in cardiomyocytes after different treatments. Left: histogram of mitochondrial NAD⁺ levels; middle: histogram of mitochondrial NADH levels; right: histogram of mitochondrial NAD⁺/NADH ratio. (e, f) Intracellular glutathione levels of cardiomyocyte after different treatments. Left: histogram of intracellular GSH levels; middle: histogram of intracellular GSSG levels; right: histogram of intracellular GSH/GSSG ratio. Data are presented as (). A: vs. control group (anoxia); B: vs. anoxia group; C: vs. Cap+anoxia group; D: vs. control group (A/R); E: vs. A/R group; F: vs. Cap+A/R group.

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