Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh Signaling

Figure 5

Nrf2 mediates ROS production induced by Cav-1 knockdown in PSCs. (a) Nrf2 protein levels in PSCs were determined by western blot. (b) GSH and GSSG levels were evaluated in PSCs (middle and right). versus the sh-Ctrl group (). All data are representative of at least three independent experiments. (c–e) Cav-1 in PSCs was silenced by transfection with control shRNA (sh-ctrl) or shRNA targeting Cav-1 (sh-Cav-1). Nrf2 overexpression DNA (Nrf2 vector) or control DNA (control vector) was added to the Cav-1-silenced group to determine the effect of Nrf2 overexpression on Cav-1-knockdown PSCs. Nrf2 and shh protein expression was detected by western blot. (f) ROS production in PSCs was evaluated with DCF-DA and normalized based on total protein content. Data represent the . denotes , by two-tailed Student’s -test.

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