Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 10

Inhibition of Nrf2 activity partly abolishes the anti-inflammatory, antiapoptotic, and mitochondrial protective role of rhSTC1. HK-2 cells were treated with iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 (50 ng/ml) or ML385 (10 μM). (a) Representative images of transmission electron microscope analysis. (b) Western blot analysis of the expression of inflammation, apoptosis, and mitochondrial damage was associated with proteins (). (c) Western blot analysis of the expression of nuclear and cytoplasm Nrf2 (). (d–l) Quantification of average Western blot band intensities. Values were presented as . , compared with the control group. ^#, compared with the iohexol group. ^▲, compared with the rhSTC1 treatment group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)