Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 4

Silencing STC1 significantly increases inflammation response and mitochondrial damage in HK-2 cells. HK-2 cells were firstly transfected with STC1-siRNA, or control siRNA, and 24 hours later, these cells were treated with iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 (50 ng/ml). Then, the whole cell lysates were collected for Western blot analysis or fixed for confocal microscopy analysis. (a) Western blot analysis of the expression of inflammation and mitochondrial damage-associated proteins (). (b) Western blot analysis of the expression of mitophagy-associated proteins (). (c) Representative images. HK-2 cells were treated as mentioned above. MitoTracker (red) was used to label mitochondria (). (d–l) Quantification of average Western blot band intensities. Values were presented as . , compared with the control group. ^#, compared with the iohexol group.

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