Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 5

Antiapoptotic effects of rhSTC1 in HK-2 cells. (a, c, and g) HK-2 cells were then exposed to iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 at different concentrations (50 ng/ml, 100 ng/ml). (b, d, and h) HK-2 cells were firstly transfected with STC1-siRNA, or control siRNA, and 24 hours later, these cells were treated with iohexol (200 mg iodine/ml) for 4 hours. (a, b) Western blot analysis of cleaved caspase 3 expression in the whole cells (). (c, d) Representative images. Cells were stained with Hoechst 33342. Cellular and nuclear morphology was recorded by phase-contrast and fluorescence microscopy. (e, f) Quantification of average Western blot band intensities. (g, h) The percentage of apoptosis was estimated by counting the cells with typical apoptotic morphology. Values were presented as . , compared with the control group. , compared with the iohexol group.

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