Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 7

rhSTC1 treatment restores the changes in Drp1 and mfn2 expression and reduces ROS production in the rat kidneys. Rats were treated as mentioned above. (a) Western blot was used to detect the expression of Drp1, mfn2, and TOMM20 (). (b) Kidney sections from the mentioned groups were stained with Drp1, mfn2, and TOMM20 antibodies for IHC analysis (magnification, ×400). Oxidative stress in kidney tubular cells was assessed by using DHE. (c–e) Quantification of average Western blot band intensities. (f) Quantification of tissues stained with DHE. (g) Plasma mitochondrial DNA Tc number decreased after iohexol treatment, which was reversed by rhSTC1. Values were presented as . , compared with the control group. ^#, compared with the CI-AKI group.

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