Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress

Figure 2

Autophagy reduces spontaneous and replication stress-induced micronuclei. (a, b) Rapa or Torin1 treatment effectively activates autophagy. HT1080 cells and U2OS cells were treated with autophagy activator Rapa (20 nM) or Torin1 (50 nM) for 24 h, and cells were harvested for measurement of autophagy efficiency (LC3 and p62) by Western blot analysis (the numbers below indicate the ratios of LC3-II/LC3-I). (c, d) Autophagy activators reduce spontaneous and replication stress-induced micronuclei. HT1080 cells and U2OS cells were treated with 20 nM Rapa and 50 nM Torin1 or cotreated with 200 μM HU for 24 h. The frequencies of MN were tested at 48 h after drugs were washed out. Each experiment was repeated three times. and . Rapa: rapamycin.

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